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Revista de Ciências Agrárias
versión impresa ISSN 0871-018X
Resumen
MARTINS, M. R.; SANTOS, F.; CANDEIAS, P. y CRUZ-MORAIS, J.. Effect of temperature, pH and Hg2+ and Pb2+ traces in dehydrogenaseand urease activities of a soil from Évora region. Rev. de Ciências Agrárias [online]. 2010, vol.33, n.1, pp.314-322. ISSN 0871-018X.
Enzyme activities are often used as indicator of soil quality. This study reports on dehydrogenase (EC 1.1.1) and urease (EC 3.5.1.5) activities of a soil under Olea europaea L. from Évora region. Kinetic constants Km and Vmax were determined using p-iodonitrotetrazolium chloride (INT) and urea, respectively. Effects of pH, temperature and Hg2+ and Pb2+ traces on both activities were determined. Maximal activity was obtained at pH = 8.5 and 40ºC, Km = 0.5 mM and Vmax=5,4µmol min-1 g-1 , for dehydrogenase and at pH = 10 and 37 ºC, Km = 25.7 mM and Vmax = 2.0x10-2 µmol min-1 g-1, for urease. These activities were inhibited by different concentrations of Hg2+, but only the urease activity was inhibited by Pb2+. Results of this study are comparable to those reported in the literature for these enzymes.
Palabras clave : Dehydrogenases; Soil enzyme; Soil quality; Urease.