Abstract

SILVA, D.; GONCALVES, M.  and  COSTA, A.. Optimization of an Epifluorescence Microscopy Technique for rapid quantification of bacteria asociated to corrosion products in produced water. Corros. Prot. Mater. [online]. 2013, vol.32, n.4, pp.105-107. ISSN 0870-1164.

Microbial cells constitute a severe problem for the petroleum industry. Among the microorganisms, the general anaerobic bacteria (GAnB) are the most common, which includes the sulfate-reducing bacteria (SRB). Nowadays, the GAnB are enumerated by the most probable number (MPN), providing the final quantification after 28 days. In this work, a method for the rapid quantification of bacteria in the produced water from oil extraction was optimized using epifluorescence microscopy with acridine orange dye. This method provided a more accurate estimate of the number of GAnB when compared with enumeration by the MPN technique. Furthermore, it is performed in just 30 minutes per sample.

Keywords : Fluorescence Microscopy; Acridine Orange; Anaerobic Bacteria; Microbial Quantification; Produced Water from Oil.

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