Abstract

VALDIVIESSO, Teresa  and  COSTA, Rita Lourenço da. Controlled Pollinations in Castanea spp. and Caracterization of the Parents and Progenies for Microsatellities . Silva Lus. [online]. 2006, vol.14, n.1, pp.23-31. ISSN 0870-6352.

This study presents the first results obtained in controlled pollinations, performed for the production of hybrid populations involving resistant (Castanea crenata Sieb. & Zucc and Castanea mollissima Blume) and susceptible (Castanea sativa Mill) genotypes to ink and blight diseases thus enabling the mapping of genes resistant to fungal diseases. The cultivars selection (the feminine parent) was targeted on the individuals with the greatest economic interest, while the choice of the masculine parents (C. crenata and C mollissima), on the diseases resistance. For the total of 68 isolations in 4 cultivars, about 408 flowers were pollinated. The total production of hybrid seeds was of 21 fruits. These seeds were then germinated, resulting in 11 plants. DNA was extracted both from the parents, as well as their progeny and nuclear microsatellites were applied. The inheritance of the loci, for the different microsatellites, involving parents and progenies, was then analysed.

Keywords : chestnut; cross-pollinations; fungal resistance.

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