Abstract

EVARISTO, Isabel; TENREIRO, Rogério  and  COSTA, Rita. Molecular Characterisation of Stone Pine's Selected Genotypes for Pine Nut Quality Control . Silva Lus. [online]. 2007, vol.15, n.2, pp.139-170. ISSN 0870-6352.

In order to assess the genetic diversity, within and among Pinus pinea populations, 25 different provenances from seven countries (Portugal, Spain, Italy, Greece, Morocco, Turkey and Israel) and seven Portuguese populations originated from three Provenance Regions were selected. These populations have been analysed using different types of molecular markers: RAPD (Random Amplified Polymorphic DNA), cpSSR (chloroplast Simple Sequence Repeat), AFLP (Amplified Fragment Length Polymorphism) and SSAP (Sequence Specific Amplification Polymorphism). In the case of cpSSR, no genetic variability was revealed since a single haplotype was obtained for all the populations analysed. However, with the other markers different levels of polymorphism were obtained. RAPD profiles revealed 52% of polymorphic fragments with 15 different primers. Our results showed that SSAP approach was more efficient to retrieve information than AFLP analysis, leading to the highest number of polymorphic fragments obtained and higher levels of estimated genetic diversity. With SSAP, 232 distinct fragments were scored, and 175 were polymorphic (75%), while with the three AFLP primer combination (EcoRI/MseI) 132 distinct fragments were produced, with 75 (57%) being polymorphic. The greater genetic variation was found mainly within populations and the fixation index F_ST averaged over loci ranged from 7% with AFLP to 11% with SSAP.

Keywords : genetic diversity; Pinus pinea; RAPD; cpSSR; AFLP; SSAP.

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