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Silva Lusitana

Print version ISSN 0870-6352

Abstract

OLIVEIRA, Maria Beatriz de; ABREU, Heber dos Santos  and  PEREIRA, Regina Paula Willemen. Tenor of Lignin in Seedlings of Eucalyptus urophylla S. T. Blake Treated with Phytoregulators. Silva Lus. [online]. 2009, vol.17, n.1, pp.51-57. ISSN 0870-6352.

Lignin is an indispensable biomacromolecule for various biological processes in plants. One of the most important lignin functions is to maintain the rigidity needed for the verticality of the main stem and to ensure the water and mineral movement. This research was carried out with seedlings of Eucalyptus urophylla and the results may facilitate further studies in biotechnology. In this research were applied different concentrations of plant growth regulators jasmonic acid (1 and 2 μM) and 2,4-dichlorophenoxyacetic acid (18.12 and 36.24 μM)]. The experiment was full factorial with a completely randomized design. The results showed that the treatment with jasmonic acid in its first dosage enabled the process of lignin formation and in a second dosage disabled the process of lignification. In the treatment with 2,4-D (A1-18.12 μ M) the process of lignification contributed to reduce the deposition of lignin, while (A2 - 36.24 μM) had a smaller reduction compared with the standard. The combined treatments J1A1 (1 μM jasmonic acid and 18.12 + μ M 2,4-D) and J1A2 (2 μ M jasmonic acid and (36.24 + μ M 2,4-D) showed synergistic effect with a predominance of jasmonic acid on lignin formation. The results pointed out for change in the lignification process as well as protein and anatomical features.

Keywords : 2,4-dichlorophenoxy acetic acid; jasmonic acid; lignification.

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