Abstract

AROKIARAJ, Mark Christopher  and  MENESSON, Eric. Novel anti-inflammatory and immunomodulation effects of Rose on the endothelium in normal and hypoxic invitro conditions. Angiol Cir Vasc [online]. 2019, vol.15, n.4, pp.238-248. ISSN 1646-706X.

Aims: The study was performed in search of a novel agent useful for inflammation modulation, and cytokine and related biomarkers levels, which would result in the treatment of cardiovascular disorders and various other clinical scenarios. Materials and methods: A crushed red rose extract was prepared from the petals, and it was processed for analysis. The extract was tested on HUVEC cells at various concentrations. By microscopic examination of cells, a safe concentration was identified, and the levels below the safe limit were tested at 72 hours and seven days for selected cytokines secretion. After hypoxia treatment, the experiment was performed at various concentrations and varying degrees of hypoxia (12%, 5%, and 1% oxygen) during treatment, and the results were compared with the controls. Results: The majority of the Inflammatory cytokine's secretion was reduced by the treatment of red rose extract on the endothelial cells. VEGF and angiogenic cytokine levels were reduced, but VEGF-R2 levels were maintained after the cell treatment. Below the safe concentration limit (0.5%), there were only minimal changes in the cytokine levels tested at various dilutions. IL 1, TNF ±, ADAM ST13, Angiopoietin levels and other inflammatory markers were reduced. In further experiments, the rose extract also induced Fas ligand, ERB4, integrin A5, Insulin R, IGF1 R, and XIAP; and reduces Trail R1, ICAM 1, and BMPR2. LDL R receptors were elevated in the endothelial cells. Conclusion There is potential for a red rose extract for the reduction of in vascular inflammatory biomarkers and certain other cytokine levels. Further studies need to be performed to evaluate the benefits and pharmacokinetics.

Keywords : Inflammation; Molecular biology; Rose.

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