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Portugaliae Electrochimica Acta

Print version ISSN 0872-1904

Port. Electrochim. Acta vol.26 no.1 Coimbra  2008

 

Acetylcholinesterase – Choline Oxidase Biosensor for Pirimicarb Determination

 

 

S.C.A. Sousa, M.J.F. Rebelo*

 

CCMM -Faculdade de Ciências da Universidade de Lisboa, Campo Grande, C 8, 1749-016 Lisboa, Portugal

 

Received 29th June 2007; accepted 27th September 2007

 

 

Abstract

A biosensor for the determination of pirimicarb was constructed by the co-immobilization of acetylcholinesterase and choline oxidase on a derivatised polyethersulfone membrane. The membrane was applied to an Universal Sensors electrode base system and the hydrogen peroxide formation was monitored upon addition of acetylcholine to an appropriately chosen buffer where the biosensor was dipped.

The hydrogen peroxide is the product of the hydrolysis of choline, catalysed by cholineoxidase. Choline was formed by the action of acetylcholinesterase on acetylcholine. Pirimicarb inhibits these reactions and that fact can be used to determine its concentration. A calibration plot for pirimicarb was obtained.

Keywords: acetylcholine, biosensor, pirimicarb, acetylcholinesterase, polyethersulfone.

 

 

Biosensor de Acetilcolinesterase – Colina Oxidase para Determinação do Pirimicarbo

Resumo

Construiu-se um biossensor para a determinação do pirimicarbo, pela co-imobilização de acetilcolinesterase (AChE) e colina oxidase (ChOx) numa membrana de poliétersulfona modificada. A membrana foi aplicada a um sistema base de eléctrodos da Universal Sensors e monitorizou-se o peróxido de hidrogénio formado quando da adição de acetilcolina (ACh) a um tampão apropriadamente escolhido onde se mergulhou o biossensor.

O peróxido de hidrogénio é o produto da hidrólise da colina, catalisada pela colineoxidase. A colina formou-se pela acção da acetilcolinesterase na acetilcolina. O pirimicarbo inibe estas reacções e esse facto pode ser usado para determinar a sua concentração. Obteve-se um gráfico de calibração para o pirimicarbo.

 

 

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References

 

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5.  http://academic.scranton.edu/faculty/CANNM1/biochemistry/biochemistrymodule.html

6.  F.N. Kok, F. Bozuglu, V. Hasirci, Bios. Bioelectr. 17 (2002) 531-539.

 

 

* Corresponding author. E-mail address: mjrebelo@fc.ul.pt

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