<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0870-6352</journal-id>
<journal-title><![CDATA[Silva Lusitana]]></journal-title>
<abbrev-journal-title><![CDATA[Silva Lus.]]></abbrev-journal-title>
<issn>0870-6352</issn>
<publisher>
<publisher-name><![CDATA[Unidade de Silvicultura e Produtos Florestais]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0870-63522005000200002</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Residues of the Cork Industry as Carriers for the Production of Legume Inoculants]]></article-title>
<article-title xml:lang="pt"><![CDATA[Resíduos da Indústria dos Aglomerados da Cortiça como Potenciais Substractos para a Produção de Inoculantes para Sementes de Leguminosas]]></article-title>
<article-title xml:lang="fr"><![CDATA[Résidus de l'Industrie du Liège comme Substrats pour la Production des Inoculums pour les Légumineuses]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ferreira]]></surname>
<given-names><![CDATA[Eugénio Mendes]]></given-names>
</name>
<xref ref-type="aff" rid="A01"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castro]]></surname>
<given-names><![CDATA[Isabel Videira e]]></given-names>
</name>
<xref ref-type="aff" rid="A01"/>
</contrib>
</contrib-group>
<aff id="A01">
<institution><![CDATA[,Estação Florestal Nacional Departamento de Ecologia, Recursos Naturais e Ambiente ]]></institution>
<addr-line><![CDATA[OEIRAS ]]></addr-line>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2005</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2005</year>
</pub-date>
<volume>13</volume>
<numero>2</numero>
<fpage>159</fpage>
<lpage>167</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_arttext&amp;pid=S0870-63522005000200002&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_abstract&amp;pid=S0870-63522005000200002&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_pdf&amp;pid=S0870-63522005000200002&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Growth and survival of two rhizobial strains, Rhizobium leguminosarum bv. trifolii, and Mesorhizobium ciceri, were used to evaluate residues from the cork industry as potential carriers for production of rhizobial inoculants. A peat-based carrier was used as a control. 41 days after inoculation, the number of viable bacteria was high, about 10(9) bacteria g-1 for all carriers. Rhizobial survival during storage (450 days) remained stable, between 10(8) and 10(9). The cork residues based carriers showed good handling properties and water holding capacities, they were non-toxic and had a nearly neutral pH. All these factors indicate that the use of these carriers is viable for the production of rhizobial inoculants.]]></p></abstract>
<abstract abstract-type="short" xml:lang="pt"><p><![CDATA[O crescimento e a sobrevivência de duas estirpes de Rhizobium, uma de Rhizobium leguminosarum bv. trifolii, e outra de Mesorhizobium ciceri, foram usados para avaliação de resíduos da indústria dos aglomerados da cortiça como potenciais substractos para a produção de inoculantes para sementes de leguminosas. A turfa foi usada como controlo. 41 dias após a inoculação, o n.º das bactérias viáveis era elevado e semelhante em todos os substractos, aproximadamente 10(9) bactérias g-1. A sobrevivência durante os 450 dias de armazenamento permaneceu estável, entre 10(8) e 10(9). Os resíduos da cortiça mostraram possuir boas características de manuseio, boas capacidades de retenção de água, não apresentaram toxicidade, possuíam pH perto da neutralidade, indicando poderem ser utilizados como substractos alternativos à turfa na produção de inoculantes para leguminosas.]]></p></abstract>
<abstract abstract-type="short" xml:lang="fr"><p><![CDATA[La croissance et la survivance de deux souches de bactéries, Rhizobium leguminosarum bv. trifolii et Mesorhizobium ciceri, ont été employées pour évaluer l'utilisation des résidus de l'industrie de la fabrication des agglomérés du liège comme potentiels substrats pour la production d'inoculants pour les semences des légumineuses. La tourbe a été utilisée comme témoin. 41 jours après inoculation, le nombre de bactéries viables était élevé et semblable pour tous les substrats, environ 10(9) bactéries g-1. La survivance pendant le stockage, 450 jours, est restée stable, entre 10(8) et 10(9). Les résidus de l'industrie du liège ont montré avoir de bonnes propriétés de manipulation et de rétention d'eau. De même ils étaient non toxiques, pouvant être employés comme substrats possibles pour la production des inoculums rhizobiens.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[carriers]]></kwd>
<kwd lng="en"><![CDATA[cork industry]]></kwd>
<kwd lng="en"><![CDATA[legume inoculants]]></kwd>
<kwd lng="en"><![CDATA[Rhizobium]]></kwd>
<kwd lng="pt"><![CDATA[inoculantes]]></kwd>
<kwd lng="pt"><![CDATA[resíduos da indústria da cortiça]]></kwd>
<kwd lng="pt"><![CDATA[Rhizobium]]></kwd>
<kwd lng="fr"><![CDATA[inoculums]]></kwd>
<kwd lng="fr"><![CDATA[liège]]></kwd>
<kwd lng="fr"><![CDATA[résidus de l'industrie]]></kwd>
<kwd lng="fr"><![CDATA[Rhizobium]]></kwd>
</kwd-group>
</article-meta>
</front><body><![CDATA[ <p align="center" class=Texto><b>Residues of the Cork Industry as Carriers for the Production      of Legume Inoculants</b></p>        <p class=MsoNormal align=center>&nbsp;</p>      <p class=MsoNormal align=center><b>Eugénio Mendes Ferreira<a href="#1">*</a><a name="top1"></a>    and Isabel Videira e Castro</b></p>      <p class=MsoNormal align=center>Senior Researchers</p>      <p class=MsoNormal align=center>Estação Florestal Nacional. Departamento de Ecologia, Recursos Naturais e Ambiente. Av. da República, Quinta do Marquês, 2780-159 OEIRAS</p>        <p>&nbsp;</p>       <p>&nbsp;</p>      <p><b>Abstract.</b> Growth and survival of two rhizobial strains, <i>Rhizobium leguminosarum</i> bv. <i>trifolii</i>, and <i>Mesorhizobium</i> <i>ciceri</i>, were used to evaluate residues from the cork industry as potential carriers for production of rhizobial inoculants. A peat-based carrier was used as a control. 41 days after inoculation, the number of viable bacteria was high, about 10<sup>9</sup> bacteria g<sup>-1 </sup>for all carriers. Rhizobial survival during storage (450 days) remained stable, between 10<sup>8</sup> and 10<sup>9</sup>. The cork residues based carriers showed good handling properties and water holding capacities, they were non-toxic and had a nearly neutral pH. All these factors indicate that the use of these carriers is viable for the production of rhizobial inoculants.</p>      <p><b>Key words</b>:<b> </b>carriers; cork industry; legume inoculants; <i>Rhizobium</i></p>         <p>&nbsp;</p>        ]]></body>
<body><![CDATA[<p><b>Resíduos da Indústria dos Aglomerados da Cortiça como Potenciais Substractos    para a Produção de Inoculantes para Sementes de Leguminosas</b></p>      <p><b>Sumário.</b> O crescimento e a sobrevivência de duas estirpes de <i>Rhizobium</i>, uma de<i> Rhizobium leguminosarum</i> bv. <i>trifolii</i>, e outra de <i>Mesorhizobium</i> <i>ciceri</i>, foram usados para avaliação de resíduos da indústria dos aglomerados da cortiça como potenciais substractos para a produção de inoculantes para sementes de leguminosas. A turfa  foi usada como controlo. 41 dias após a inoculação, o n.º das bactérias viáveis era elevado e semelhante em todos os substractos, aproximadamente 10<sup>9</sup> bactérias g<sup>-1</sup>. A sobrevivência durante os 450 dias de armazenamento  permaneceu  estável, entre 10<sup>8</sup> e 10<sup>9</sup>. Os resíduos da cortiça mostraram possuir boas características de manuseio, boas capacidades de retenção de água, não apresentaram toxicidade, possuíam pH perto da neutralidade, indicando poderem ser utilizados como substractos alternativos à turfa na produção de inoculantes para leguminosas..</p>      <p><b>Palavras-chave</b>: inoculantes; resíduos da indústria da cortiça ; <i>Rhizobium</i></p>        <p>&nbsp;</p>      <p><b>Résidus de l'Industrie du Liège comme Substrats pour la Production des Inoculums pour les Légumineuses</b><b></b></p>      <p><b>Résumé. </b>La croissance et la survivance de deux souches de bactéries, <i>Rhizobium leguminosarum</i> bv. <i>trifolii</i> et <i>Mesorhizobium ciceri</i>, ont été employées pour évaluer l'utilisation des résidus de l'industrie de la fabrication des agglomérés du liège comme potentiels substrats pour la production d'inoculants pour les semences des légumineuses. La tourbe a été utilisée comme témoin. 41 jours après inoculation, le nombre de bactéries viables était élevé et semblable pour tous les substrats, environ 10<sup>9 </sup>bactéries g<sup>-1</sup>. La survivance pendant le stockage, 450 jours, est restée stable, entre 10<sup>8</sup> et 10<sup>9</sup>. Les résidus de l'industrie du liège ont montré avoir de bonnes propriétés de manipulation et de rétention d'eau. De même ils étaient non toxiques, pouvant être employés comme substrats possibles pour la production des inoculums rhizobiens.</p>      <p><b>Mots clés</b>: inoculums;<i> </i>liège;<i> </i>résidus de    l'industrie;<i> Rhizobium</i></p>     <p>&nbsp;</p>     <p>&nbsp;</p>     <p>Texto completo disponivel apenas em PDF.</p>     ]]></body>
<body><![CDATA[<p>Full text only available in PDF format.</p>         <p><u></u></p>     <p>&nbsp;</p>      <p><b>References </b></p>         <!-- ref --><p>BOONKERD, N., 1991. Inoculant quality control and standards in Thailand    In <i>Report on the Expert Consultation on Legume Inoculants Production and    Quality Control,</i> FAO, Rome, pp. 121-129.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000027&pid=S0870-6352200500020000200001&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>BROCKWELL, J., BOTTOMLEY, P.J., 1995. Recent advances in inoculant technology and prospects for the future. <i>Soil Biology &amp; Biochemistry</i> <b>27 </b>: 683-697.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000028&pid=S0870-6352200500020000200002&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>CATROUX, G., 1991. Inoculant quality standards and controls in France.    In <i>Report on the Expert Consultation on Legume Inoculants Production and    Quality Control</i>, FAO, Rome, pp. 113-120.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000029&pid=S0870-6352200500020000200003&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>CATROUX, G., HARTMAN, A., REVELIN, C., 2001. Trends in rhizobial inoculant production and use. <i>Plant and Soil</i>  <b>230 </b>: 21-30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000030&pid=S0870-6352200500020000200004&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>DAZA, A., SANTAMARIA, C., RODRIGUEZ-NAVARRO, D.N., CAMACHO, M., ORIVE, R., TEMPRANO, F., 2000. Perlite as a carrier for bacterial inoculants. <i>Soil Biology &amp; Biochemistry</i> <b>32 </b>: 567-572.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000031&pid=S0870-6352200500020000200005&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>EINNARSSON, S., GUDMUNDSSON, J., SVERRISSON, H., KRISTJANSSON, J.K., RUNOLFSSON, S., 1993. Production of <i>Rhizobium</i> inoculants for <i>Lupinus nootkatensis</i> on nutrient-supplemented pumice. <i>Applied and Environmental Microbiology</i>  <b>59 </b>: 3666-3668.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000032&pid=S0870-6352200500020000200006&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>GRIFFITH, G.W., ROUGHLEY R.J., 1992. The effect of moisture potential on growth and survival of root nodule bacteria in peat culture and on seed. <i>Journal of Applied Bacteriology</i> <b>73</b> : 7-13. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000033&pid=S0870-6352200500020000200007&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>SMITH, R.S., 1987. Production and quality control of inoculants. In <i>Symbiotic Nitrogen Fixation Technology</i>, Gerald H., Elkan, Marcel Dekker, New York, pp. 392-411.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000034&pid=S0870-6352200500020000200008&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>SMITH, R.S., 1992. Legume inoculant formulation and application. <i>Canadian Journal of Microbiology</i> <b>38 </b>: 485-492.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000035&pid=S0870-6352200500020000200009&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>STEPHENS, J.H.G., RASK, H., 2000. Inoculant production and formulation. <i>Field Crops Research</i> <b>65 </b>: 249-258.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000036&pid=S0870-6352200500020000200010&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><!-- ref --><p>STRIJDOM, B.W., DESCHODT, C.C., 1976. Carriers of rhizobia and the effect of prior treatment on the survival of rhizobia. 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Blackwell, Oxford, 164 pp.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000039&pid=S0870-6352200500020000200013&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><p class=MsoBodyText>&nbsp;</p>     <p class=MsoBodyText>&nbsp;</p>      <p><i>Entregue para publicação em Agosto de 2004</i></p>      ]]></body>
<body><![CDATA[<p><i>Aceite para publicação em Março de 2005</i></p>        <p>&nbsp;</p>    <b> </b>      <p><a name="1"></a><a href="#top1">*</a> 1&ordm; Autor E-mail:<a href="mailto:eugeniomferreira@net.sapo.pt">eugeniomferreira@net.sapo.pt</a></p>         ]]></body><back>
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