Proteomics and ingestive behaviour

E. Lamy¹, F.Capela-Silva^1,2, E.S. Baptista^1,3, G. Graça⁴, G.Costa⁴ & A.V. Coelho^4,5

RESUMO

O comportamento ingestivo é regulado, entre outros, por factores afectivos, os quais determinam diferentes preferências. A escolha da dieta depende de experiências anteriores, bem como do nível de percepção gustativa, estando a saliva envolvida neste último. As proteínas salivares desempenham papéis muito diversos que incluem a lubrificação, digestão, protecção contra microorganismos e factores antinutritivos, e têm um papel decisivo na transdução do gosto. A nossa abordagem ao estudo do comportamento ingestivo utiliza a proteómica para identificar e monitorizar a expressão das proteínas salivares. A palavra proteómica é utilizada para descrever o estudo do proteoma, ou seja, a medição simultânea da totalidade de proteínas de uma matriz biológica. Temos como objectivo contribuir para uma melhor compreensão dos mecanismos envolvidos na regulação do comportamento em pastoreio, através do estudo do proteoma salivar de cabras e ovelhas, cujas preferências gustativas diferem.

ABSTRACT

Ingestion behavior is controlled, among others, by affective factors, which lead to different preferences. Diet selection depends on animal past experience as well as taste perception, in which saliva is involved. Salivary proteins have diverse functions, which include lubrication, digestion, protection against microorganisms and antinutritional compounds and have an important role in taste transduction. Our approach to the study of feeding behavior uses proteomics to access expression profile and to identify salivary proteins. The word proteomics is used to describe the study of proteome, i.e. the simultaneous measurement of the entirety of proteins from a biological matrix. We aim to contribute to a better understanding of grazing and browsing control mechanisms through the study of sheep and goats salivary proteome, species that show different diet preferences.

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REFERÊNCIAS BIBLIOGRÁFICAS

Aardema, M.J. & MacGregor, J.T. 2002. Toxicology and genetic toxicology in the new era of "toxicogenomics": impact of "-omics" technologies. Mutat Res, 499: 13-25.         [ Links ]

Amado, F.M., Vitorino, R.M., Domingues, P.M., Lobo, M.J. & Duarte, J.A. 2005. Analysis of the human saliva proteome. Expert Rev Proteomics, 2: 521-539.

Amasaki, T., Amasaki, H. & Nagasao, J. 2001. Immunohistochemical localization of carbonic anhydrase isoenzymes in salivary glands and intestine in adult and suckling pigs. J Vet Med Sci, 63: 1147-1149.

Baxter, N.J., Lilley, T.H., Haslam, E. & Williamson, M.P. 1997. Multiple interactions between polyphenols and salivary proline-rich protein repeat result in complexation and precipitation. Biochemistry, 36: 5566-5577.

Bedi, G.S. 1991. The effect of adrenergic agonists and antagonists on cysteine-proteinase inhibitor (cystatin) in rat saliva. Arch Oral Biol, 36: 611-618.

Burgess, S.C. 2004. Proteomics in the chicken: tools for understanding immune responses to avian diseases. Poult Sci, 83: 552-573.

Christensen, C.M. & Navazesh, M. 1984. Anticipatory salivary flow to the sight of different foods. Appetite, 5:307-315.

de Venevelles, P., Chich, J.F., Faigle, W., Loew, D., Labbe, M., Girard-Misguich, F. & Pery, P. 2004. Towards a reference map of Eimeria tenella sporozoite proteins by two-dimensional electrophoresis and mass spectrometry. Int J Parasitol, 34: 1321-1331.

Dhingra, V., Gupta, M., Andacht, T. & Fu, Z.F. 2005. New frontiers in proteomics research: a perspective. Int J Pharm, 299: 1-18

Duffy, V.B. & Bartoshuk, L.M. 2000. Food acceptance and genetic variation in taste. J Am Diet Assoc, 100: 647-55

Friedman, D.B., Hill, S., Keller, J.W., Merchant, N.B., Levy, S.E., Coffey, R.J. & Caprioli, R.M. 2004. Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics, 4: 793-811.

Fuchs, D., Winkelmann, I., Johnson, I.T., Mariman, E., Wenzel, U. & Daniel, H. 2005. Proteomics in nutrition research: principles, technologies and applications. Br J Nutr, 94: 302-314.

Hagerman, A.E. & Robbins, C.T. 1993. Specificity of tannin-binding salivary proteins relative to diet selection by mammals. Can J Zool, 71: 628-633

Hamler, R.L., Zhu, K., Buchanan, N.S., Kreunin, P., Kachman, M.T., Miller, F.R. & Lubman, D.M. 2004. A – dimensional liquid-phase separation method coupled with mass spectrometry for proteomic studies of breast cancer and biomarker identification. Proteomics, 4: 562-577.

Huang, C.M. 2004. Comparative proteomic analysis of human whole saliva. Arch Oral Biol. 49: 951-62

Hu, S., Xie, Y., Ramachandran, P., Ogozalek Loo, R.R., Li, Y., Loo, J.A., & Woong, D.T. 2005. Large-scale identification of proteins in human salivary proteome by liquid chromatography/mass spectrometry and two-dimensional gel electrophoresis-mass spectrometry. Proteomics, 5: 174-28

Ishihara, K., Nakata, K., Yamagishi, N., Iwasaki, S., Kiriike, N. & Hatayama, T. 2005. A comparative proteomic analysis of the rat brain during rebound hyperagia induced by space-restrition. Mol Cell Biochem, 276: 21-29.

Katsukawa et al. 1999 Induction of salivary gurmarin-binding proteins in rats fed gymnema-containing diets. Chem. Senses, 24: 387-392.

Kay, R.N.B, Engelhardt, W.V. & White, R.G. 1980. The digestive physiology of wild ruminants. In: The Digestive Physiology and Metabolism in Ruminants, 36: 743-761. Ruckebusch, Y. & Thivend, P. (Ed.) MTP Press Limited, Lancaster, UK.

Kojima, T., Andersen, E., Sanchez, J.C., Wilkins, M.R., Hochstrasser, D.F., Pralong, W.F. & Cimasoni, G. 2000. Human gingival crevicular fluid contains MRP8 (S100A8) and MRP14 (S100 A9), two calcium-binding proteins of the S100 family. J Dent Res, 79: 740-7.

Lamy, E., da Costa, G., Coelho A.V., Capela e Silva, F. & Sales Baptista, E. (2003) The effect of polyphenols in mammalian herbivores saliva composition. 9th International European Association for Veterinary Pharmacology and Toxicology Congress. 14-17 July, Lisboa, Portugal. (Poster G-34).

Lindemann, B. 2001. Receptores and transduction in taste. Nature, 413: 219-225.

Lu, Y. & Bennick, A. 1998. Interaction of tannin with human salivary proline-rich proteins. Arch Oral Biol, 43: 717-728.

Martin, S. & Pangborn, R.M. 1971. Human parotid secretion in response to ethyl alcohol. J Dent Res, 50: 485-490.

Matsuo, R. 2000. Role of saliva in the maintenance of taste sensitivity. Crit Rev Oral Biol Med, 11: 216-229.

Miller, I.J. Jr. & Whitney, G. 1989. Sucrose octaacetate-taster mice have more vallate taste buds than non-tasters. Neurosci Lett, 100: 271-5

Narauto, N., Wong, P., Lu, Y., Wroblewski, K. & Bennick, A. 1999. Interaction of tannin with human salivary histatins. J Agric Food Chem, 47: 2229-2234.

Noble, A.C. 1995. Application of time-intensity procedures for the evaluation of taste and mouthfeel. Am J Enol Vitic, 46:128-133.

Provenza, F.D. 1995. Postingestive feedback as an elementary determinant of food preference and intake in ruminants. J Range Manage, 48: 2-17.

Richardson, C.T. & Feldman, M. 1986. Salivary response to food in humans and its effect on gastric acid secretion. Am J Physiol, 250:G85-G91.

Scott, E.M., Carter, A.M. & Findlay, J.B. 2005. The application of proteomics to diabetes. Diab Vasc Dis Res, 2: 54-60.

Strzezek, J., Wysocki, P., Kordan, W. & Kuklinska, M. 2005. Proteomics of boar seminal plasma current studies and possibility of their application in biotechnology of animal reproduction. Reprod Biol, 5: 279-290.

Villalba, J.J. & Provenza, F.D. 2005. Foraging in chemically diverse environments: energy, protein and alternative foods influence ingestion of plant secundary metabolites by lambs. J Chem Ecol, 31: 123-138.

Williams, K.M., Ekström, J. & Marshall, T. 1999a. High-resolution electrophoretic analysis of rat parotid salivary proteins. Electrophoresis, 20: 1373-1381.

Williams, K.M., Ekström, J. & Marshall, T. 1999b The protein composition of ferret parotid saliva as revealed by high-resolution electrophoretic methods. Electrophoresis, 20: 2818-23.

Wroblewski, K., Muhandiram, R., Chakrabartty, A. & Bennick, A. 2001. The molecular interaction of human salivary histatins with polyphenolic compounds. Eur J Biochem, 268: 4384-4397.

Yan, Q. & Bennick, A.1995. Identification of histatins as tannin-binding proteins in human saliva. Biochem J, 311: 341-347.

¹ ICAM - Instituto de Ciências Agrárias Mediterrânicas, Universidade de Évora, 7002-554 Évora, e-mail: ecsl@uevora.pt; ²Departamento de Biologia, Universidade de Évora; ³Departamento de Zootecnia, Universidade de Évora; ⁴ITQB - Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa