<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0871-018X</journal-id>
<journal-title><![CDATA[Revista de Ciências Agrárias]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. de Ciências Agrárias]]></abbrev-journal-title>
<issn>0871-018X</issn>
<publisher>
<publisher-name><![CDATA[Sociedade de Ciências Agrárias de Portugal]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0871-018X2007000100047</article-id>
<title-group>
<article-title xml:lang="pt"><![CDATA[Actividade antimicrobiana do óleo essencial do Foeniculum vulgare Miller]]></article-title>
<article-title xml:lang="en"><![CDATA[Antimicrobial activity of Foeniculum vulgare Miller essential oil]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Tinoco]]></surname>
<given-names><![CDATA[M. T.]]></given-names>
</name>
<xref ref-type="aff" rid="A01"/>
<xref ref-type="aff" rid="A02"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Martins]]></surname>
<given-names><![CDATA[M. R.]]></given-names>
</name>
<xref ref-type="aff" rid="A01"/>
<xref ref-type="aff" rid="A02"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cruz-Morais]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<xref ref-type="aff" rid="A01"/>
<xref ref-type="aff" rid="A02"/>
</contrib>
</contrib-group>
<aff id="A01">
<institution><![CDATA[,Universidade de Évora Departamento de Química ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<aff id="A02">
<institution><![CDATA[,Universidade de Évora Instituto de Ciências Agrárias Mediterrânicas (ICAM) ]]></institution>
<addr-line><![CDATA[Évora ]]></addr-line>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>01</month>
<year>2007</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>01</month>
<year>2007</year>
</pub-date>
<volume>30</volume>
<numero>1</numero>
<fpage>448</fpage>
<lpage>454</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_arttext&amp;pid=S0871-018X2007000100047&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_abstract&amp;pid=S0871-018X2007000100047&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_pdf&amp;pid=S0871-018X2007000100047&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="pt"><p><![CDATA[O Foeniculum vulgare Mill. subsp. vulgare é uma planta espontânea da região mediterrânea, pertencente à família Apiaceae. O seu óleo essencial, principalmente o dos frutos secos, é amplamente utilizado nas indústrias alimentar, farmacêutica, cosmética e perfumaria. Neste trabalho, pretendeu-se avaliar a actividade antimicrobiana do óleo essencial dos frutos verdes e das folhas da variedade de funcho doce colhido no Alentejo, na região de Évora, e relacionar essa actividade com a respectiva composição química. A extracção dos óleos essenciais foi efectuada por hidrodestilação e a sua análise foi feita por GC-FID e GC-MS. No óleo das folhas foram identificados como componentes maioritários os fenilpropanóides anetol, fenchona e estragol e o monoterpeno a-felandreno, enquanto que o óleo dos frutos apresentou como constituintes predominantes anetol e fenchona. A actividade antimicrobiana dos óleos foi avaliada face às estirpes Staphylococcus aureus, Escherichia coli, Saccharomyces spp., Fusarium oxysporum e Penicillium sp.. Os óleos essenciais apresentaram actividade antimicrobiana contra S. aureus, Saccharomyces spp. e Fusarium oxysporum.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Foeniculum vulgare Mill. ssp. vulgare is a spontaneous plant of Mediterranean region that belongs to the Apiaceae family. Its essential oil is used as additives in food, pharmaceutical, cosmetic and perfume industries mainly that one obtained from dried seeds. The main goal of this work were to evaluate the antimicrobial activity of essential oils obtained from fresh leaves and unripe seeds of sweet fennel, collected in Évora-Alentejo, in face of their chemical composition. The extraction of the essentials oils was made by hydrodistillation. Chemical analyses were carried out by GC-FID and GCMS. Phenylpropanoides anethole, fenchone, estragole and the monoterpene aphellandrene were the most abundant compounds of leaves essential oil. Seeds essential oil showed as main components anethole and fenchone. Antimicrobial activities were tested against the strains Staphylococcus aureus, Escherichia coli, Saccharomyces spp., Fusarium oxysporum and Penicillium sp.. Seed and leaves essential oils showed antimicrobial activity against S. aureus Saccharomyces spp. and Fusarium oxysporum.]]></p></abstract>
</article-meta>
</front><body><![CDATA[ <p align="center"><b>Actividade antimicrobiana do óleo essencial do <i>Foeniculum    vulgare</i> Miller </b></p>     <p align="center"><b>Antimicrobial activity of <i>Foeniculum vulgare</i> Miller    essential oil </b></p>     <p>&nbsp;</p>     <P align="center">M. T. Tinoco<Sup><a href="#1">1</a><a name="top1"></a></Sup>,    M. R. Martins<Sup><a href="#1">1</a><a name="top1"></a></Sup> &amp; J. Cruz-Morais<Sup><a href="#1">1</a><a name="top1"></a>    </Sup></P>     <p>&nbsp;</p>     <p align="center"><b>RESUMO </b></p>     <p>O <i>Foeniculum vulgare</i> Mill. subsp. <i>vulgare</i> é uma planta espontânea    da região mediterrânea, pertencente à família Apiaceae. O seu óleo essencial,    principalmente o dos frutos secos, é amplamente utilizado nas indústrias alimentar,    farmacêutica, cosmética e perfumaria. </P>     <p>Neste trabalho, pretendeu-se avaliar a actividade antimicrobiana do óleo essencial    dos frutos verdes e das folhas da variedade de funcho doce colhido no Alentejo,    na região de Évora, e relacionar essa actividade com a respectiva composição    química. </P>     <p>A extracção dos óleos essenciais foi efectuada por hidrodestilação e a sua    análise foi feita por GC-FID e GC-MS. No óleo das folhas foram identificados    como componentes maioritários os fenilpropanóides anetol, fenchona e estragol    e o monoterpeno a-felandreno, enquanto que o óleo dos frutos apresentou como    constituintes predominantes anetol e fenchona. </P>     <p>A actividade antimicrobiana dos óleos foi avaliada face às estirpes <i>Staphylococcus    aureus, Escherichia coli, Saccharomyces</i> spp., <i>Fusarium oxysporum e Penicillium</i>    sp.. Os óleos essenciais apresentaram actividade antimicrobiana contra <i>S.    aureus, Saccharomyces</i> spp. e <i>Fusarium oxysporum</i>. </P>     ]]></body>
<body><![CDATA[<p>&nbsp;</p>     <p align="center"><b>ABSTRACT </b></p>     <p><i>Foeniculum vulgare</i> Mill. ssp. <i>vulgare</i> is a spontaneous plant    of Mediterranean region that belongs to the Apiaceae family. Its essential oil    is used as additives in food, pharmaceutical, cosmetic and perfume industries    mainly that one obtained from dried seeds. </P>     <p>The main goal of this work were to evaluate the antimicrobial activity of essential    oils obtained from fresh leaves and unripe seeds of sweet fennel, collected    in Évora-Alentejo, in face of their chemical composition. </P>     <p>The extraction of the essentials oils was made by hydrodistillation. Chemical    analyses were carried out by GC-FID and GCMS. Phenylpropanoides anethole, fenchone,    estragole and the monoterpene aphellandrene were the most abundant compounds    of leaves essential oil. Seeds essential oil showed as main components anethole    and fenchone. </P>     <p>Antimicrobial activities were tested against the strains <i>Staphylococcus    aureus, Escherichia coli, Saccharomyces </i>spp., <i>Fusarium oxysporum</i>    and <i>Penicillium</i> sp.. </P>     <p>Seed and leaves essential oils showed antimicrobial activity against <i>S.    aureus Saccharomyces</i> spp. and <i>Fusarium oxysporum</i>. </P>     <p>&nbsp;</p>     <p>Texto completo disponível apenas em PDF.</p>     <p>Full text only available in PDF format.</p>     ]]></body>
<body><![CDATA[<p>&nbsp;</p>     <p align="center"><b>REFERÊNCIAS BIBLIOGRÁFICAS </b></p>     <!-- ref --><p>Adams R. P. 1995. <i>Identification of essential oil components by gaschromatography-mass    spectroscopy.</i> Allured Publishing Corporation, Illinois, USA. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=000025&pid=S0871-018X200700010004700001&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --><p>Bilia, A. R., Flamini, G., Taglioli, V., Morelli, I. &amp; Vincieri, F. F.    2002. GC-MS analysis of essential oil of some commercial Fennel teas. <i>Food    Chemistry</i>, <b>76</b>: 307-310. </P>     <p>Curtis, O. F., Shetty, K., Cassagnol, G., &amp; Peleg, M. 1996. Comparison    of synthetic and lethal effects of synthetic versions of plant metabolites (anethole,    eugenol, carvacrol, thymol) on food spoilage yeast (<i>Debaromyces hanenei</i>).    <i>Food Biotechnology</i>, <b>10</b>: 55–73. </P>     <p>De, M., De, A. K., Sen, P., &amp; Banerjee, A. B. 2002. Antimicrobial properties    of star anise (<i>Illicium verum</i> Hook f). <i>Phytotherapy Research</i>,    <b>16</b>: 94–95. </P>     <p>Duru, M.E., Öztürk, M., Ugur, A. &amp; Özgür, C. 2004. The constituents of    essential oil and in vitro antimicrobial activity of <i>Micromericia cilicica</i>    from Turkey. <i>Journal of Ethnopharmacology</i>, <b>94</b>: 43-48. </P>     <p>Farmacopeia Portuguesa VII. 2002. Vol. 2, pp 175-176. Infarmed, Lisboa. </P>     <p>Font-Quer, P.1993. <i>Plantas Medicinales, El Dioscórides Renovado</i>, Tomo    II, pp. 498-500. Editorial Labor S.A., Barcelona. </P>     <p>Karaman, S., Digrak, M., Ravid, U. &amp; Ilcim, A. 2001. Antibacterial and    antifungal activity of the essential oils of <i>Thymus revolutus</i> Celak from    Turkey. <i>Journal of Ethnopharmacology</i>, <b>76</b>: 183-186. </P>     ]]></body>
<body><![CDATA[<p>Muckensturm, B., Foechterlen, D., Reduron, J. P., Danton, P. &amp; Hildenbrand,    M. 1997. Phytochemical and Chemotaxonomic Studies of <i>Foeniculum vulgare.    Biochemical Systematics and Ecology</i>, <b>25</b>: 353-358. </P>     <p>NCCLS 1997. Reference method for broth dilution antifungal susceptibility testing    of yeasts. Approved standard M27-A. National Committee for Clinical Laboratory    Standards, Wayne, Pa. </P>     <p>NCCLS 1998. Reference method for broth dilution antifungal susceptibility testing    of conidium-forming filamentous fungi. Proposed standard M38-P. Wayne, Pa: National    Committee for Clinical Laboratory Standards; 1998. </P>     <p>Ostad, S. N., Soodi, M., Shariffzadeh, M., Khorshidi, N. &amp; Marzban, H.    2201. The effect of fennel essential oil on uterine contraction as a model for    dysmenorrhea, pharmacology and toxicology study. <i>Journal of Ethnopharmacology</i>,    <b>76</b>: 299-304. </P>     <p>Pauli, A. 2001. Antimicrobial properties of essential constituents. <i>International    Journal of Aromatherapy</i>, <b>11</b>: 126-133. </P>     <p>Proença-da-Cunha, A. Silva, A. P. &amp; Roque, O. R. 2003. <i>Plantas e Produtos    Vegetais em Fitoterapia.</i> Fundação Calouste Gulbenkian, Lisboa. </P>     <p>Roque, O.R. &amp; Proença-da-Cunha, A. 1989. Composição do óleo essencial de    <i>Foeniculum vulgare</i> Miller, espontâneo do Algarve. <i>Bol. Fac. Farm.    Coimbra</i>, <b>13</b>: 45-52. </P>     <p>Ruberto, G., Baratta, M. T., Deans, S.G. &amp; Dorman, H. J. D. 2000. Antioxidant    and antimicrobial activity of <i>Foeniculum vulgare</i> and <i>Crithmum maritimum</i>    essential oils. <i>Planta Medica</i>, <b>66</b>: 687-693. </P>     <p>Tinoco, M. T., Martins, M. R. &amp; Cruz-Morais, J. 2005. Actividade antimicrobiana    do óleo essencial do <i>Foeniculum vulgare</i> Miller. <i>Livro de Resumos -    Jornadas ICAM</i>, Évora, Portugal, p.143. </P>     <p>&nbsp;</p>     ]]></body>
<body><![CDATA[<p><Sup><a href="#top1">1</a><a name="1"></a> </Sup>Departamento de Química e    Instituto de Ciências Agrárias Mediterrânicas (ICAM), Universidade de Évora;    Apartado 94, 7002-554 Évora; e-mail: <a href="mailto:mtft@evora.pt">mtft@evora.pt</a>  </P>      ]]></body><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Adams]]></surname>
<given-names><![CDATA[R. P.]]></given-names>
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</person-group>
<source><![CDATA[Identification of essential oil components by gaschromatography-mass spectroscopy]]></source>
<year>1995</year>
<publisher-loc><![CDATA[^eIllinois Illinois]]></publisher-loc>
<publisher-name><![CDATA[Allured Publishing Corporation]]></publisher-name>
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</back>
</article>
