Fungos do género Raffaelea (Ascomycota: Ophiostomatales) associados a Platypus cylindrus (Coleoptera: Platypodidae) em Portugal

Maria Lurdes Inácio¹, Joana Henriques¹, Arlindo Lima², Edmundo Sousa¹

ABSTRACT

In the study of the fungi associated to Platypus cylindrus, several fungi were isolated from the insect and its galleries in cork oak, among which three species of Raffaelea. Morphological and cultural characteristics, sensitivity to cycloheximide and genetic variability had been evaluated in a set of isolates of this genus. On this basis R. ambrosiae and R. montetyi were identified and a third taxon segregated witch differs in morphological and molecular characteristics from the previous ones. In this work we present and discuss the parameters that allow the identification of specimens of the three taxa. The role that those ambrosia fungi can have in the cork oak decline is also discussed taking into account that Ophiostomatales fungi are pathogens of great importance in trees, namely in species of the genus Quercus.

Key-words: Ambrosia beetle, ambrosia fungi, cork oak, decline.

RESUMO

No estudo dos fungos associados ao insecto xilomicetófago Platypus cylindrus foram isolados, a partir do insecto e das suas galerias no sobreiro, diversos fungos, entre os quais três espécies de Raffaelea. Avaliaram-se características morfológicas e culturais, sensibilidade à ciclohexamida e variabilidade genética num conjunto de isolados do género. Foram identificados R. ambrosiae e R. montetyi e segregou-se um terceiro táxone que difere em características morfológicas e moleculares dos dois anteriores. No presente trabalho são apresentados e discutidos os parâmetros que permitem identificar espécimes dos três táxones. É ainda discutido o papel que estes fungos ambrósia podem ter no declínio do sobreiro, sabido que fungos Ophiostomatales são patogénios de grande importância em plantas lenhosas, nomeadamente em espécies do género Quercus.

Palavras-chave: Declínio, fungo ambrósia, insecto ambrósia, sobreiro.

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¹ Instituto Nacional de Recursos Biológicos, I.P. Edifício da ex-Estação Florestal Nacional, Quinta do Marquês, 2780-159 Oeiras lurdes.inacio@efn.com.pt ; joana.henriques@efn.com.pt; edmundo.sousa@efn.com.pt; arlindo.lima@isa.utl.pt

Comunicação apresentada no 5º Congresso da Sociedade Portuguesa de Fitopatologia, Coimbra, 2007

Recepção/Reception: 2008.02.19 aceitação/acception: 2008.08.09