<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0871-018X</journal-id>
<journal-title><![CDATA[Revista de Ciências Agrárias]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. de Ciências Agrárias]]></abbrev-journal-title>
<issn>0871-018X</issn>
<publisher>
<publisher-name><![CDATA[Sociedade de Ciências Agrárias de Portugal]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0871-018X2022000300381</article-id>
<article-id pub-id-type="doi">10.19084/rca.28478</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Biochemical and microbiological soil effects of a biostimulant based on Bacillus licheniformis-fermented sludge]]></article-title>
<article-title xml:lang="es"><![CDATA[Efectos bioquímicos y microbiológicos en el suelo de un bioestimulante a base de lodos fermentados de Bacillus licheniformis]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Caballero]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castaño]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Macías]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Martín]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Tejada]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Parrado]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,University of Seville Dept. Biochemistry and Molecular Biology ]]></institution>
<addr-line><![CDATA[Seville ]]></addr-line>
<country>Spain</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,University of Seville E.T.S.I.A. Department Crystallography, Mineralogy and Agricultural Chemistry]]></institution>
<addr-line><![CDATA[Seville ]]></addr-line>
<country>Spain</country>
</aff>
<pub-date pub-type="pub">
<day>30</day>
<month>12</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>30</day>
<month>12</month>
<year>2022</year>
</pub-date>
<volume>45</volume>
<numero>4</numero>
<fpage>381</fpage>
<lpage>390</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_arttext&amp;pid=S0871-018X2022000300381&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_abstract&amp;pid=S0871-018X2022000300381&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_pdf&amp;pid=S0871-018X2022000300381&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract A fermentative process has been applied to carry out the conversion of sludge from slaughterhouse wastewater treatment into a biostimulant complex. The three biostimulant components are: a) The Bacillus licheniformis biomass, which is a plant growth promoter rhizobacteria (PGPR), and the organism in charge of the fermentative process, b) the enzymatic secretion of said microorganism, mainly peptidases and amidases (50%), involved in N metabolism, and glucanases (33.3%), involved in carbohydrate metabolism, and c) the hydrolyzed sludge organic matter, largely composed of peptides and free aminoacids. The biostimulant has been evaluated in soil at the biochemical and microbiological level. It induced a strong microbial stimulation of the soil, and produced the specific stimulation of PGPR soil bacteria, which was studied through a metabarcoding. Moreover, mainly when applying the soluble fraction of the biostimulant complex, it was found that the Bacillus licheniformis inoculum remains active in the environment at the end of the experiment.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Se ha aplicado un proceso fermentativo para llevar a cabo la conversión de lodos procedentes de la depuración de aguas residuales de matadero en un complejo bioestimulante. Los componentes bioestimulantes son: a) la biomasa de Bacillus licheniformis, que es una rizobacteria promotora del crecimiento vegetal (PGPR), y organismo encargado del proceso fermentativo, b) la secreción enzimática de dicho microorganismo, principalmente peptidasas y amidasas (50% ), implicadas en el metabolismo del N, y las glucanasas (33,3%), implicadas en el metabolismo de los hidratos de carbono, y c) la materia orgánica del lodo hidrolizado, compuesta en gran parte por péptidos y aminoácidos libres. El bioestimulante ha sido evaluado en suelo a nivel bioquímico y microbiológico. Indujo una fuerte estimulación microbiana del suelo y produjo la estimulación específica de las bacterias del suelo PGPR, que fue estudiada a través de un metabarcode. Además, principalmente al aplicar la fracción soluble del complejo bioestimulante, se encontró que el inóculo de Bacillus licheniformis permanece activo en el ambiente al final del experimento.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Protein hydrolysate]]></kwd>
<kwd lng="en"><![CDATA[metabarcoding]]></kwd>
<kwd lng="en"><![CDATA[PGPR]]></kwd>
<kwd lng="en"><![CDATA[enzymes]]></kwd>
<kwd lng="en"><![CDATA[biodiversity]]></kwd>
<kwd lng="es"><![CDATA[Hidrolizado protéico]]></kwd>
<kwd lng="es"><![CDATA[metabarcoding]]></kwd>
<kwd lng="es"><![CDATA[PRPR]]></kwd>
<kwd lng="es"><![CDATA[enzimas]]></kwd>
<kwd lng="es"><![CDATA[biodiversidad]]></kwd>
</kwd-group>
</article-meta>
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