<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0871-018X</journal-id>
<journal-title><![CDATA[Revista de Ciências Agrárias]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. de Ciências Agrárias]]></abbrev-journal-title>
<issn>0871-018X</issn>
<publisher>
<publisher-name><![CDATA[Sociedade de Ciências Agrárias de Portugal]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0871-018X2022000301031</article-id>
<article-id pub-id-type="doi">10.19084/rca.28746</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Remediation of a chlorpyrifos contaminated soil using novel bacterial strains and cyclodextrin: evaluation of its effectiveness by ecotoxicity studies]]></article-title>
<article-title xml:lang="es"><![CDATA[Recuperación de un suelo contaminado con clorpirifós usando nuevas cepas bacterianas y ciclodextrina: evaluación de su efectividad a través de estudios de ecotoxicidad]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Lara-Moreno]]></surname>
<given-names><![CDATA[Alba]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morillo]]></surname>
<given-names><![CDATA[Esmeralda]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Merchán]]></surname>
<given-names><![CDATA[Francisco]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Madrid]]></surname>
<given-names><![CDATA[Fernando]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Villaverde]]></surname>
<given-names><![CDATA[Jaime]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Spanish National Research Council Institute of Natural Resources and Agrobiology of Seville Department of Agrochemistry, Environmental Microbiology and Soil Conservation]]></institution>
<addr-line><![CDATA[Seville ]]></addr-line>
<country>Spain</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,University of Seville Faculty of Pharmacy Department of Microbiology and Parasitology]]></institution>
<addr-line><![CDATA[Seville ]]></addr-line>
<country>Spain</country>
</aff>
<pub-date pub-type="pub">
<day>30</day>
<month>12</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>30</day>
<month>12</month>
<year>2022</year>
</pub-date>
<volume>45</volume>
<numero>4</numero>
<fpage>1031</fpage>
<lpage>1040</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_arttext&amp;pid=S0871-018X2022000301031&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_abstract&amp;pid=S0871-018X2022000301031&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_pdf&amp;pid=S0871-018X2022000301031&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Chlorpyrifos (CLP) is one of the most widely used insecticides in the world. However, it is highly toxic for living organisms and persistent in the environment. Biological treatment is considered as a good option to remediate polluted environmental areas. Two bacterial strains, Bacillus megaterium CCLP1 and Bacillus safensis CCLP2 were isolated from two selected agricultural soils (R and LL), using enrichment technique in presence of CLP as only carbon and energy source. These strains were able to remove in solution up to 99.1 and 98.9% of CLP after 60 d (initial concentration: 10 mg L-1). Several treatments were performed in a soil artificially contaminated with CLP to enhance its remediation: 1. biostimulation, adding micro- and macronutrients; 2. bioaugmentation, inoculating B. megaterium CCLP1 or B. safensis CCLP2; 3. Addition of randomly methylated &#946;-cyclodextrin (RAMEB), as bioavailability enhancement; 4. bioaugmentation + RAMEB. The best CLP biodegradation results were achieved when bioaugmentation and RAMEB were jointly applied. After biodegradation treatment, an ecotoxicological test was carried out to verify the effectiveness of the bioremediation strategy in soil. Results pointed out that bacteria individually inoculated into the soil were able to decrease the toxicity to undetectable levels.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Clorpirifos (CLP) es uno de los insecticidas más usados en el mundo. Sin embargo, es altamente tóxico para los organismos vivos y persistente en el medio ambiente. El tratamiento biológico es considerado como una buena opción para recuperar áreas contaminadas. Dos cepas bacterianas, Bacillus megaterium CCLP1 y Bacillus safensis CCLP2 fueron aisladas a partir de dos suelos agrícolas a través de cultivos de enriquecimientos en presencia de CLP como única fuente de carbono y energía. Estas cepas fueron capaces de eliminar un 99.1 y 98.9% de CLP en solución tras 60 d de ensayo (concentración inicial: 10 mg L-1). Varios tratamientos fueron aplicados en un suelo artificialmente contaminado con CLPpara su biorrecuperación: 1. bioestímulo, adicionando macro- y micronutrientes; 2. bioaumento, inoculando B. megaterium CCLP1 o B. safensis CCLP2; 3. Adición de &#946;-ciclodextrina aleatoriamente metilada (RAMEB), como potenciador de la biodisponibilidad; 4. bioaumento + RAMEB. Los mejores resultados de biodegradación de CLP fueron obtenidos cuando bioaumento y RAMEB fueron aplicados conjuntamente. Tras el tratamiento de biodegradación, un ensayo ecotoxicológico fue realizado para verificar la efectividad de la estrategia de biorrecuperación en el suelo. Los resultados mostraron que las bacterias inoculadas individualmente en el suelo fueron capaces de reducir la toxicidad a niveles indetectables.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Chlorpyrifos]]></kwd>
<kwd lng="en"><![CDATA[bioremediation]]></kwd>
<kwd lng="en"><![CDATA[soil]]></kwd>
<kwd lng="en"><![CDATA[Bacillus]]></kwd>
<kwd lng="en"><![CDATA[cyclodextrin]]></kwd>
<kwd lng="es"><![CDATA[Clorpirifos]]></kwd>
<kwd lng="es"><![CDATA[biorecuperación]]></kwd>
<kwd lng="es"><![CDATA[suelo]]></kwd>
<kwd lng="es"><![CDATA[Bacillus]]></kwd>
<kwd lng="es"><![CDATA[ciclodextrina]]></kwd>
</kwd-group>
</article-meta>
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