<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0871-018X</journal-id>
<journal-title><![CDATA[Revista de Ciências Agrárias]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. de Ciências Agrárias]]></abbrev-journal-title>
<issn>0871-018X</issn>
<publisher>
<publisher-name><![CDATA[Sociedade de Ciências Agrárias de Portugal]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0871-018X2024000400011</article-id>
<article-id pub-id-type="doi">10.19084/rca.38525</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Unveiling of beneficial bacteria in Douro Vineyards]]></article-title>
<article-title xml:lang="pt"><![CDATA[Pesquisa de bactérias benéficas nas Vinhas do Douro]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Erbiai]]></surname>
<given-names><![CDATA[El Hadi]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Alves]]></surname>
<given-names><![CDATA[Fernando]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Marques]]></surname>
<given-names><![CDATA[Guilhermina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,University of Trás-os-Montes and Alto Douro Department of Agronomy Centre for the Research and Technology of Agro-Environmental and Biological Sciences]]></institution>
<addr-line><![CDATA[Vila Real ]]></addr-line>
<country>Portugal</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Symington Family Estates  ]]></institution>
<addr-line><![CDATA[Vila Nova de Gaia ]]></addr-line>
<country>Portugal</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,UTAD Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production ]]></institution>
<addr-line><![CDATA[Vila Real ]]></addr-line>
<country>Portugal</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2024</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2024</year>
</pub-date>
<volume>47</volume>
<numero>4</numero>
<fpage>11</fpage>
<lpage>20</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_arttext&amp;pid=S0871-018X2024000400011&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_abstract&amp;pid=S0871-018X2024000400011&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.pt/scielo.php?script=sci_pdf&amp;pid=S0871-018X2024000400011&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Microbial biodiversity in soil vineyards is a key determinant of grapevine (Vitis vinifera L.) growth and wine quality. Vineyards are subject to many pests and fungal pathogens responsible for significant losses in the wine sector. Recently, beneficial bacteria have received increasing attention as a promising sustainable alternative to chemical fertilizers and pesticides. This work aimed to isolate and characterize bacteria, with biofertilizer and biopesticide potential, from vineyards in the Douro Wine Region. The isolates were tested for their antagonistic activity against 4 grapevine phytopathogenic fungi. Thirty antagonistic isolates were identified by 16S rDNA sequences and subjected to the test of their plant growth-promoting properties. Identification results revealed the presence of 7 bacteria genera, mainly Bacillus species. Strains of Burkholderia sp. (Gs3) and Streptomyces sp. showed the highest antifungal effect. Concerning biofertilizer potential, all isolates produced IAA (values between 1 and 53 &#120583;g/mL). The strain Gs3 was the highest producer of IAA and the most powerful phosphate solubilizer. For siderophores, Bacillus spp. were the main producing strains. Therefore, it can be suggested that these bacteria can be an alternative to agrochemicals. However, further studies are required to evaluate the properties of these bacteria in field conditions, separately and/or in combination, aiming to develop commercial products.]]></p></abstract>
<abstract abstract-type="short" xml:lang="pt"><p><![CDATA[Resumo A biodiversidade microbiana no solo das vinhas é determinante do crescimento da videira (Vitis vinífera L.) e da qualidade do vinho. As videiras estão sujeitas a diversas pragas e doenças responsáveis por perdas significativas no sector vitivinícola. Recentemente, as bactérias têm recebido uma atenção crescente como alternativa sustentável e promissora aos fertilizantes e pesticidas químicos. Este trabalho teve como objetivo isolar e caracterizar bactérias, com potencial biofertilizante e biopesticida, provenientes de vinhas da região do Douro. Os isolados foram testados para as atividades antagonistas contra 4 fungos fitopatogénicos da videira. Trinta isolados antagonistas foram identificados por análise das sequências da região 16S do rDNA e submetidos à avaliação das suas propriedades promotoras do crescimento de plantas. Os resultados revelaram a presença de 7 géneros de bactérias, predominando as espécies de Bacillus. Estirpes de Burkholderia sp. (Gs3). e Streptomyces sp. apresentaram o maior efeito antifúngico. Quanto ao potencial biofertilizante, todos os isolados produziram IAA. A estirpe Gs3 foi a maior produtora de IAA e o mais eficiente solubilizador de fosfato. As espécies do género Bacillus destacaram-se na produção de sideróforos. Assim, pode concluir-se que estas bactérias podem ser uma alternativa aos agroquímicos. No entanto, são necessários mais estudos para avaliar as suas propriedades em condições de campo, isoladamente e/ou em combinação, visando o desenvolvimento de produtos comerciais.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Phytopathogenic fungi]]></kwd>
<kwd lng="en"><![CDATA[biocontrol]]></kwd>
<kwd lng="en"><![CDATA[biofertilizer]]></kwd>
<kwd lng="en"><![CDATA[grapevine]]></kwd>
<kwd lng="en"><![CDATA[beneficial bacteria]]></kwd>
<kwd lng="pt"><![CDATA[fungos fitopatogénicos]]></kwd>
<kwd lng="pt"><![CDATA[biocontrolo]]></kwd>
<kwd lng="pt"><![CDATA[biofertilizante]]></kwd>
<kwd lng="pt"><![CDATA[videira]]></kwd>
<kwd lng="pt"><![CDATA[bactérias benéficas]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Akhtar]]></surname>
<given-names><![CDATA[M.S.]]></given-names>
</name>
<name>
<surname><![CDATA[Siddiqui]]></surname>
<given-names><![CDATA[Z.A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Role of Plant Growth Promoting Rhizobacteria in Biocontrol of Plant Diseases and Sustainable Agriculture]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Maheshwari]]></surname>
<given-names><![CDATA[D.K.]]></given-names>
</name>
</person-group>
<source><![CDATA[Plant Growth and Health Promoting Bacteria]]></source>
<year>2010</year>
<volume>18</volume>
<page-range>157-95</page-range><publisher-loc><![CDATA[Berlin, Heidelberg ]]></publisher-loc>
<publisher-name><![CDATA[Springer Berlin Heidelberg]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Alexander]]></surname>
<given-names><![CDATA[D.B.]]></given-names>
</name>
<name>
<surname><![CDATA[Zuberer]]></surname>
<given-names><![CDATA[D.A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Use of Chrome Azurol S Reagents to Evaluate Siderophore Production by Rhizosphere Bacteria]]></article-title>
<source><![CDATA[Biology and Fertility of Soils]]></source>
<year>1991</year>
<volume>12</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>39-45</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Costa]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Fraga]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Malheiro]]></surname>
<given-names><![CDATA[A.C.]]></given-names>
</name>
<name>
<surname><![CDATA[Santos]]></surname>
<given-names><![CDATA[J.A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Application of Crop Modelling to Portuguese Viticulture: Implementation and Added-Values for Strategic Planning]]></article-title>
<source><![CDATA[Ciência e Técnica Vitivinícola]]></source>
<year>2015</year>
<volume>30</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>29-42</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Grassi]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
<name>
<surname><![CDATA[De Lorenzis]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Back to the Origins: Background and Perspectives of Grapevine Domestication]]></article-title>
<source><![CDATA[International Journal of Molecular Sciences]]></source>
<year>2021</year>
<volume>22</volume>
<numero>9</numero>
<issue>9</issue>
<page-range>4518</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kumar]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Stecher]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Li]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Knyaz]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Tamura]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms]]></article-title>
<source><![CDATA[Molecular Biology and Evolution]]></source>
<year>2018</year>
<volume>35</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>1547-9</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Laranjeira]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Fernandes-Silva]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Reis]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Torcato]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Raimundo]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
<name>
<surname><![CDATA[Ferreira]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Carnide]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[Marques]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Inoculation of Plant Growth Promoting Bacteria and Arbuscular Mycorrhizal Fungi Improve Chickpea Performance under Water Deficit Conditions]]></article-title>
<source><![CDATA[Applied Soil Ecology]]></source>
<year>2021</year>
<volume>164</volume>
<page-range>103927</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Mehta]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Nautiyal]]></surname>
<given-names><![CDATA[C.S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[An Efficient Method for Qualitative Screening of Phosphate-Solubilizing Bacteria]]></article-title>
<source><![CDATA[Current Microbiology]]></source>
<year>2001</year>
<volume>43</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>51-6</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Patten]]></surname>
<given-names><![CDATA[C.L.]]></given-names>
</name>
<name>
<surname><![CDATA[Glick]]></surname>
<given-names><![CDATA[B.R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Role of Pseudomonas putida Indoleacetic Acid in Development of the Host Plant Root System]]></article-title>
<source><![CDATA[Applied and Environmental Microbiology]]></source>
<year>2002</year>
<volume>68</volume>
<numero>8</numero>
<issue>8</issue>
<page-range>3795-801</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ranjbariyan]]></surname>
<given-names><![CDATA[A.R.]]></given-names>
</name>
<name>
<surname><![CDATA[Shams-Ghahfarokhi]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Kalantari]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Razzaghi-Abyaneh]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Molecular Identification of Antagonistic Bacteria from Tehran Soils and Evaluation of Their Inhibitory Activities toward Pathogenic Fungi]]></article-title>
<source><![CDATA[Iranian Journal of Microbiology]]></source>
<year>2011</year>
<volume>3</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>140-6</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Suleimanova]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Bulmakova]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Sokolnikova]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Egorova]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<name>
<surname><![CDATA[Itkina]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Kuzminova]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Gizatullina]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Sharipova]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Phosphate Solubilization and Plant Growth Promotion by Pantoea Brenneri Soil Isolates]]></article-title>
<source><![CDATA[Microorganisms]]></source>
<year>2023</year>
<volume>11</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>1136</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wang]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Ren]]></surname>
<given-names><![CDATA[X.]]></given-names>
</name>
<name>
<surname><![CDATA[Su]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Han]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Contributions of Beneficial Microorganisms in Soil Remediation and Quality Improvement of Medicinal Plants]]></article-title>
<source><![CDATA[Plants]]></source>
<year>2022</year>
<volume>11</volume>
<numero>23</numero>
<issue>23</issue>
<page-range>3200</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
